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Phototaxis assay. All non-AS1-treated fish significantly chose the light side of the arena, whereas all AS1-treated fish significantly preferred the dark, regardless of whether 1 uM naloxone was applied. N = 39 fish for the DMSO condition, 41 fish for the 2.5 uM AS1 condition, 41 fish for 1 uM naloxone, and 39 fish for 1 uM naloxone + 2.5 uM AS1.
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