Bar Plot from Scientific Research

Temperature choice assay (28.5 °C vs 37.5 °C) with naloxone, an opioid receptor antagonist. Naloxone does not appear to replicate or attenuate the effects of AS1; both control and naloxone only treated fish significantly choose the 28.5 °C side of the arena, whereas AS1 only and Naloxone + AS1-treated fish significantly choose the 37.5 °C side. N = 37, 56, 52, and 45 fish for DMSO, 1 μM Naloxone, 5 μM AS1, and 1 μM Naloxone + 5 μM AS1 conditions, respectively.

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