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AS1 dose–response curve in the non-sensitized thermal preference assay (28.5 vs 37.5 °C). At low concentrations, AS1 does not influence preference for 28.5 °C, whereas intermediate concentrations (1.25 μM) eliminate preference for either zone, and high concentrations (> 2.5 μM) induce preference for the 37.5 °C zone. N = 58, 58, 60, 63, 63, 62 for 0, 0.25, 0.5, 1.25, 2.5, and 5 μM AS1 conditions, respectively.
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