Thermal preference assay assessing choice between rearing temperature (28.5 °C) and a range of aversive temperatures (31.5 °C, 33.5 °C, 35.5 °C, and 37.5 °C). As the intensity of the heat stimulus increases, vehicle-treated fish increasingly prefer the 28.5 °C zone, while the AS1-treated fish increasingly prefer the hot zone. All DMSO-treated fish significantly preferred the 28.5 °C, whereas all AS1-treated fish significantly preferred the 37.5 °C side, regardless of temperature comparison. N = 117 fish for DMSO and 127 for AS1 (31.5 °C), 111 for DMSO and 126 for AS1 (33.5 °C), 105 for DMSO and 125 for AS1 (35.5 °C), 73 for DMSO and 124 for AS1 (37.5 °C). * represents significant differences from 31.5 °C DMSO condition, whereas # represents significant differences from the 31.5 °C AS1 condition.
