Gel from Scientific Research

Open access visualization of Gel, Western Blotting, Bacterial Josephins, HEK293T Cell Lysates, BpJOS
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DOI: 10.1016/j.molcel.2025.02.002

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Analysis of bacterial Josephins cleavage products by western blotting. HEK293T cell lysates were incubated with BpJOS, BcJOS, PaJOS, HeJOS, or PcJOS for 1 h. Substrate deubiquitination and mono-ubiquitin accumulation are shown by α-ubiquitin detection and diGly-remnant accumulation by α-K-ε-GlyGly detection. α-actin staining served as loading control. Asterisks mark unspecific bands.

#Gel#Western Blotting#Bacterial Josephins#HEK293T Cell Lysates#BpJOS#BcJOS#PaJOS#HeJOS#PcJOS#Substrate Deubiquitination#Mono-Ubiquitin#α-Ubiquitin#diGly-remnant#α-K-ε-GlyGly#α-Actin

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