Fluorescence anisotropy of NRAS-NanoLuc mRNA-Cy5 (~720 bases, 230 kDa) as function of DDX3X(WT) (pink), DDX3X(D354V) (blue), and DDX3X(G325E) (gray) concentration w/o (left) and w/ (right) ATP in binding buffer. Solid line depicts model fit to derive the apparent KD(dashed line) for each DDX3X variant.
