Impact of Endotoxin (LPS) on BRITE Adipocyte Response to Adrenergic Stimulus. Lean ( A1, B1 ) and obese ( A2, B2 ) primary human adipocytes were differentiated with or without 2 uM rosiglitazone (Rosi), 100 ng/mL lipopolysaccharide (LPS100) or a combination of the two. When fully differentiated, cells were treated with 10 uM isoproterenol (Iso). BAT genes uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1alpha) were analyzed using RT-PCR with L19 as a housekeeping control. Data are presented as mean +/- SEM. Two-way ANOVA was used to test significance; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 compared to Iso treatment; f p < 0.05, ff p < 0.01, fff p < 0.001, ffff p < 0.0001 compared to Rosi + Iso treatment
