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RT-qPCR analysis (bottom left) and proliferation (bottom right) of 786-O CRISPRi cells expressing the indicated sgRNAs and a dox-inducibleCRNDEUCE-containing isoform rescue construct. 0.08 g/mL of dox was used for the induction ofCRNDEUCE. The positions of RT-qPCR amplicons (A–C) are indicated in the transcript schematic. Transcript abundance was normalized toGAPDH.
#Bar Plot#Error Bars#Line Plot#RT-qPCR#Cell Proliferation#CRISPRi#sgRNAs#Doxycycline#CRNDEUCE#786-O Cells#GAPDH
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