FRAP analysis of G3BP1-mRNA-Cy5 condensates in FRAP buffer. Top: Frames depicting a condensate before (left), directly after (middle), and 300 s after photobleaching (right). Red circle depicts photobleached area. Bottom: FRAP analysis of GFP-G3BP1 (black, mean, SD,n= 34) and mRNA-Cy5 (purple, mean, SD,n= 22) pooled from two independent experiments. Initial slopes determined by linear regression of the first 5 s for G3BP1 and 50 s for mRNA-Cy5.
