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On the innermost ring, the amino acid at position 91 of GyrA is coded by colour. On the second ring, the amino acid at position 95 of GyrA is also coded by colour. The ciprofloxacin MIC of each isolate is shown on the third ring in red, and isolates with GyrA 91S and GyrA 95N/G/A are shown on the outermost ring in black. Isolates from this study are marked by arrows. Tree scale represents the recombination-adjusted number of single-nucleotide polymorphisms. MIC=minimum inhibitory concentration.

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