Representative images (E–G) and quantification (H–J) of MBP, AcTub, and FIGQY antibody labeling (with colabeling of the postsynaptic density by MAGUK) in WT, nsfaI209N/st53, and nsfast53 larvae. Images are maximum intensity z projections from the initial segment of the posterior lateral-line nerve. Quantification data are shown as mean ± SEM; p values were determined by Student’s t tests to compare with WT group. ns, not significant;*p < 0.05,**p < 0.01,***p < 0.001. For all experiments, n ≥ 5 fish per genotype (5 dpf). All images and data are representative of 2 or 3 independent experiments. Scale bar, 10 μm.
