Bar Plot from Scientific Research

Lipid-dependent oxygen consumption in prostate organoids generated from Pten−/−and Pten−/−;AmpkACTmice aged 1 year in the presence and absence of exogenous palmitate (PAL; 150 μM). The oxygen consumption rate (OCR) was measured on an Agilent Seahorse XFe96 analyzer before and after addition of etomoxir (100 μM). Lipid-dependent O2consumption (%) was calculated per well as (baseline OCR – etomoxir OCR)/baseline OCR) × 100. Data from two independent organoid preparations from different mice are shown. Data are means ± SEM. n = 10–14 organoids (wells) per genotype. One-way ANOVA with Tukey’s post hoc test was used to determine significant differences between genotypes;*p < 0.05,***p < 0.005,****p < 0.001.

DOI