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In-cell deubiquitination assays. 293T shOTUD3#1 cells were cotransfected with FLAG-cGAS, HA-OTUD3 WT/CA, and Myc-Ub (WT or mutant). FLAG-cGAS was purified in denaturing conditions after cells were treated with MG132. The samples were analyzed by Myc-Ub WB.
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