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Tyrosine deprivation decreased apoptosis in AC-16 cells and ACMs, measured by flow cytometry. Cells were treated with tyrosine-deficient medium for 2 h and 500 μM H2O2 for 1 h before harvesting (n = 4 biological replicates). Data are expressed as mean ± SEM. Nonparametric two-tailed Student’s t test was used to compare each group. Significance was indicated asnsp > 0.05,*p < 0.05,**p < 0.01, and***p < 0.001. See alsoFigure S3.
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