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AREG-stimulated DVL3 cells were analyzed after trametinib treatment: (-) vehicle (DMSO), (+) 25 nM, or (++) 50 nM. Immunoblot analysis of phosphorylation of ERK1/2 (pERK), EGR1 and vinculin loading control 24 h post-trametinib. Quantification of pERK/ERK for the indicated treatments. Data represent at least three independent experiments (mean SD). Significance, unpaired t test.
#Bar Plot#Error Bars#Gel#AREG-Stimulated DVL3 Cells#Trametinib Treatment#Immunoblot Analysis#Phosphorylation of ERK1/2#EGR1#Vinculin#pERK/ERK Quantification
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