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Relative RNA expression ofAregandEgr1measured by RT-qPCR after silencingEgr1(siEgr1) in PCa luminal cells for 7 days. Non-targeting siRNAs (siControl). B-actin was used for normalization. Data represent three independent experiments (mean ± SD). Two-way ANOVA, Dunnett’s multiple comparisons test was used. EGR1 protein was measured by western blot. Vinculin, loading control.
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