Scatter Plot from Scientific Research

Overlay of the1H-15N-HSQC spectrum of 250 μM15N-labeled BTN3A1-IgV (black), with 250 μM15N-labeled BTN3A1-IgV after the addition of 250 μM of WT and different mutants of BTN2A1-IgV (colored as indicated). Most resonances of BTN3A1-IgV are unaffected, but some (highlighted here by K136) show significant line broadening consistent with being in intermediate chemical exchange leading to the loss of signal. Mutants of BTN2A1-IgV cause varying effects on the1H-15N-HSQC spectrum of BTN3A1-IgV suggesting differential binding effects.

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