Microscopy from Scientific Research

Maximum intensity projections (D, dorsal view) and single cross-sections (S, sagittal view) of MZoep (B and C), cycmorphant (MO, 4 ng) (E and F), and slb/wnt11f2 (H and I) gsc::caax-EGFP embryos at 6 hpf (B, E, and H) and 8 hpf (C, F, and I); for control embryos see (Figures 1B and 1C); dashed orange lines outline the YSL-DC boundary (S, sagittal view) in MZoep embryos; dashed white lines outline the ppl and, due to the leaky nature of this transgenic reporter line, also the posterior axial mesendoderm (pam); dashed blue lines outline the IF at the EVL-DC boundary; dashed pink lines outline the IF at the YSL-DC boundary (S, sagittal view); scale bars, 100 μm.

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