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Schematic illustrating the principle of ppl animal-directed velocity quantification; AV, animal-vegetal axis. (D') Animal-directed velocities of individual ppl cells in control (green, n = 5) and ouabain-treated embryos (blue, n = 4); mean SEM. (D'') Representative three-dimensional ppl cell tracks (7.5 hpf) in control (green) and ouabain-treated (blue)gsc::caax-EGFPembryos at 7.5 hpf; sagittal views.
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