Microscopy from Scientific Research

Maximum intensity projections (D, dorsal view) and single cross-sections (S, sagittal view) ofgsc::caax-EGFPembryos expressing EGFP within the axial ME and injected with fluorescent dextran to mark IF in ouabain-treated and mannitol-injected embryos at 6 hpf dashed white lines outline the anterior axial mesendoderm/prechordal plate (ppl) and, due to the leaky nature of this transgenic reporter line, also the posterior axial ME (pam); dashed blue lines outline the IF at the EVL-DC boundary; dashed pink lines outline the IF at the YSL-DC boundary (S, sagittal view); scale bars, 100 μm.

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