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Timeline of functional birthdating experiments. Larvae were birthdated at either 30 or 48 hpf and then imaged on a confocal (4 dpf) and two-photon (5 dpf) to identify projection neuron soma position, birthdate, and directional (up/down) identity. Projection neurons were visualized using theTg(-6.7Tru.Hcrtr2:GAL4-VP16)line and expressed both the UAS:Kaede and UAS:GCaMP6s indicators.
#Workflow#Flowchart#Functional Birthdating#Larvae#Confocal Microscopy#Two-Photon Microscopy#Projection Neuron#Soma Position#Birthdate#Directional Identity#UAS:Kaede#UAS:GCaMP6s Indicators
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