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Timeline of topography validation experiments. TIPM was performed on non-birthdated larvae at 5 dpf as shown inFigure 2B, using either a SCAPE or two-photon microscope. Projection neurons were visualized using theTg(-6.7Tru.Hcrtr2:GAL4-VP16)line and expressed only the UAS:GCaMP6s indicator.
#Illustration#Workflow#Topography Validation#TIPM#Larvae#SCAPE Microscopy#Two-Photon Microscopy#Projection Neurons#UAS:GCaMP6s Indicator
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