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Cell-free IRF3 dimerization assay, comparing the activity of the mitochondrial fraction isolated from WT, G3BPs, and G3BPs/MAVS U2OS cells. Data are presented in means ± SD. p values were calculated using two-tailed unpaired Student’s t test (ns, p > 0.05). RNA-seq results contain 2 biological repeats and were confirmed by two independent experiments. All other data are representative of at least three independent experiments.). Raw data for the heatmap can be found in the supplemental file (Data S1,S2, andS3).
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