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Activation state of MAVS, as measured by cell-free IRF3 dimerization assay. Mitochondrial fraction (P5) containing MAVS was isolated from U2OS cells 6 h post-dsRNA, and mixed with a common pool of cytosolic extract (S18) from unstimulated WT U2OS cells and in vitro-translated35S-IRF3. Dimerization of35S-IRF3 was analyzed by native gel assay.*indicates mini-MAVS.

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