Left panel: basal reporter expression changes during iPSC to iNeuron differentiation across 3 time points grouped into 4 groups. Median with 95% confidence interval is illustrated. Group 1: turned off (one-way paired ANOVA, p = 0.0388). Group 2: constitutively active (one-way paired ANOVA, p = 0.3608). Group 3: turned on (one-way paired ANOVA, p = 0.0260). Group 4: constitutively silenced (one-way paired ANOVA, p = 0.0956). Right panel: CRISPR activation efficacy across the 4 groups at both iPSC and iNeuron stages. Violin plot shows the data distribution and the median. Fold changes of means and significance are shown (paired t test, ns: non-significant,*p < 0.05,**p < 0.01,***p < 0.001). Heatmap shows reporter activation in the 4 groups at iPSC and iNeuron stages.
