Scatter Plot from Scientific Research
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FACS determined sgRNA efficacy in human iPSCs and induced iNeurons using the reporter vector. Transfection controls demonstrate a 41.9% or 45.6% transfection efficiency in hiPSCs and iNeurons, respectively. Relative to a mix of two non-targeting sgRNAs, a mix of the five targeting sgRNAs (sgRNA 26 on F) shows 32.6x and 6.2x activation of the Venus reporter in iPSCs and iNeurons, respectively. See alsoFigure S1.
#Scatter Plot#Bar Plot#FACS#sgRNA efficacy#Human iPSCs#Induced iNeurons#Reporter vector#Transfection efficiency#Non-targeting sgRNAs#Targeting sgRNAs#Venus reporter
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