Microscopy from Scientific Research

We transferred P14 WTTcf7gfpinto WT recipients followed by LCMV infection. Adjacent sections from spleens collected on day 1 and day 2 were stained for IL-33 for computer-based definition of the IL-33 zone (H, zone definition transferred to aligned adjacent section in I and J) and for GFP (Tcf7gfp), B220 and IFN-alpha (I–K), respectively. Computer-recognized GFP+(green) and IFN-alpha+(red) cells in (I) and (J) are shown with outlines. Scale bars: 500 μm in (H) and (I); 200 μm in (J); and 100 μm in (K). IFR, interfollicular region.

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