Gel from Scientific Research

qPCR and WB analysis showed the mRNA and protein levels of SMAD7 in Calu3 and HCC827 cells with indicated treatments. V or shV represents control vectors for overexpression or shRNA knockdown, respectively. For aforementioned WB, qPCR, ChIP-qPCR and BSP assays, cells were treated with TGF-1 at final concentration of 5 ng/mL for 48 h before the indicated assays were performed ( b – i ). Error bars represent means SD derived from three independent experiments. Two-way ANOVA multiple comparison analysis was used for statistical analysis. ** P < 0.01; * P < 0.05; ns, not significant, P > 0.05.

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