Gel from Scientific Research
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Western blotting (WB) and qPCR analyses showed the effect of silencing DNMT3A and DNMT3B on the protein and mRNA level of SMAD7 in A549 and HCC827 cells with indicated treatments. For aforementioned WB, qPCR and BSP assays, cells were treated with TGF-1 at final concentration of 5 ng/mL for 48 h before the indicated assays were performed.
#Gel#Western Blotting#qPCR Analyses#DNMT3A#DNMT3B#Protein Level#mRNA Level#SMAD7#A549 Cells#HCC827 Cells#TGF-1
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