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The left sub-panel shows the genetic organizations of the SARS-CoV-2, SARS-CoV-2 GFP/ΔN, and related variants. The ORF encoding the N protein is replaced with GFP. The right sub-panel illustrates the concept that the SARS-CoV-2 GFP/ΔN virus can only replicate in lungs that are expressing the N protein.
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