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The genetic sequences encoding the human ACE2 and SARS-CoV-2 N genes were inserted together into intron 1 of the ROSA26 site located on chromosome 6. The PGK-Neo-6×SV40 pA sequence flanked by two loxP sites. When there were both the Cre recombinase and loxP sequences, the sequence between two loxP sites (PGK-Neo-6×SV40 pA) will be removed.
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