Pooled DNA-SUBs were administered to CRC lung tumour-bearing BALB/c mice (tumour, T) and saline-injected control animals (sham, S) at day 11 or 21 after tumour initiation. Urine samples were collected at 1 h after sensor administration. Cas12a trans -cleavage assays were performed against each DNA barcode and the initial reaction velocities ( V 0 ) were calculated and normalized to those of saline-injected control animals (day 11, n = 10 mice per tumour group; day 21, n = 8 mice per tumour group; n = 8 mice per control group; mean ± s.e.m.; unpaired two-tailed Mann–Whitney test, day 11 * P = 0.0343 for PAP7; day 21 * P = 0.0379 for PAP7; unpaired two-tailed t -test with Welch’s correction, ** P = 0.0054 for PAP9; * P = 0.0171 for PAP15).
