Illustration from Scientific Research

Schematic showing generation of the DNA-encoded protease-activatable nanobody (1). Disease-specific protease activity triggers (2) release of ssDNA activator into urine for disease detection (3). Study time course of urine testing and detection of the ssDNA activator with Cas12a trans -cleavage assay using fluorescent or paper readout (4). t i , time of sensor injection; t u , time of urine collection.

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