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leucine (two-way ANOVA, with Fisher’s LSD comparisons;n= 6 for WT-chow,n= 7, 6 for DIO that received VEH vs. LEP,n= 9 for OB-chow,n= 10 for OB-HFD). Two-way ANOVA with Fisher’s LSD comparisons. All error bars represent mean ± SEM. ns, not significant,*p< 0.05,**p< 0.01,***p< 0.001,****p< 0.0001.
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