ROC curve (left) and PRC (right) showing the performance of the ABC model and promoter ChIPseq intensity of the indicated histone marks in discriminating CBP/p300dependent and independent genes. CBP/p300dependent (positive list) and CBP/p300independent genes (negative list) were defined by nascent transcription analyses after acute CBP/p300 inhibition by A485 (ref. 21). Genes downregulated (twofold or greater; average fold change of 30 min, 1 h and 2 h treatment; Methods) after A485 treatment are considered CBP/p300dependent; unaffected genes (<1.2fold change) are considered CBP/p300independent. Performance was evaluated using cumulative nominal ABC scores or ChIPseq enrichment of H3K4me3, H3K4me1, H3K27ac and H2BNTac in promoter regions (within 1 kb from the TSS). The ABC score was calculated using ATACseq, H3K27ac and HiC contact frequency as reported by Fulco et al. 44.
