The H2BNTac signal is higher in distal candidate enhancers than in active promoters. Shown are the relative ChIP signal intensities of the H3K27ac and H2BNTac sites in H3K27ac-occupied regions. H3K27ac peak regions are grouped into the following categories: all, all peaks; intergenic, peaks occurring outside promoters and gene bodies; gene body (transcribed genes, TPM ≥ 2); peaks occurring within actively transcribed gene bodies; gene body (nontranscribed genes); peaks occurring within the nontranscribed gene body. Active promoters were defined as in a . The dotted lines indicate the median ratio at intergenic and active promoter regions. The box plots display the median, upper and lower quartiles; the whiskers show 1.5× IQR. Two-sided Mann–Whitney U -test, adjusted for multiple comparisons using the Benjamini–Hochberg method; NS, not significant, P ≥ 0.05, * P < 0.05, ** P < 1 × 10 −10 , *** P < 1 × 10 −50 .
