Workflow from Scientific Research
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To distinguish real mutations from damaged bases or polymerase errors, CODEC physically links Watson and Crick strands of each original duplex, which may have an alteration confined to one strand. Each cluster reads an NGS library molecule with sequences of both strands to trace a whole duplex, hence single duplex sequencing.
#Workflow#Illustration#Mutations#Damaged Bases#Polymerase Errors#Watson-Crick Strands#NGS Library#Duplex Sequencing
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