Heat map of summed basecalling error of oxidative stress relative to WT, for each nucleotide (x axis) and for each tRNA (y axis, ordered from most to least abundant in descending order). See also Supplementary Table 17. The positions of specific RNA modifications in each tRNA are listed in Supplementary Table 18. Nucleotides with a lower summed basecalling error frequency relative to WT are in blue tones, and those with a higher summed basecalling error frequency are in red tones, as seen with the terminal A at position 76 (black arrowhead). Heat maps corresponding to other biological replicates can be found in Supplementary Fig. 2b.