Microscopy from Scientific Research

Isotropic FIB-SEM image volumes (4 nm voxel size) of Drosophila brains expressing 1M-Qt FLAG-NLS (cyan) targeted to the nucleus. EMcapsulins and nuclear membranes were manually segmented and rendered within the FIB-SEM volume bounded by the ortho-slices. The magnifications (right) show ortho-slices through three EMcapsulins. Volume acquisition was performed with an SEM beam voltage of 1.3 kV and a working distance of 5 mm, at a nominal voxel size of 4 nm using an InLens detector. The FIB Ga beam was accelerated by 30 kV voltage at a current of 700 pA. Scale bars, 500 nm (overview) and 50 nm (zoom-ins). Please also see Supplementary Video 6.

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