Microscopy from Scientific Research

TEM micrographs of the identical sample from a Drosophila line with pan-neuronal expression of 1M-Mx FLAG-NLS after a standard fixation and staining protocol. Ultrathin sections were captured either on TEM grids or on silica wafers for subsequent analysis by TEM and SEM (inverted contrast), allowing for the analysis of similar cuts through the identical cell with both techniques. Corresponding TEM images were acquired on a Zeiss Libra120 at 120 kV, 13 A and 100 rad. Insets show averages ( n = 30) of the respective particle from manual segmentation. White arrowheads indicate the presence of EMcapsulin particles inside the nucleus.

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