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Open access visualization of Workflow, Illustration, EMcapsulin Monomers, Ribosomal rt Cassettes, Stop Codons
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Control over the ratios of different EMcapsulin monomers can be achieved via tunable ribosomal rt cassettes encoded on a single cistron. To this end, different combinations of stop codons and short rt-promoting motifs are combined at the end of the ORF encoding 1M-Qt. In case rt occurs, the C-terminus of 1M-Qt FLAG is further extended by an anti-GFP intrabody.

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