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To trace JCF cells, epicardioids differentiated from AAVS1-FRT-flanked STOP-mKate2-HA reporter hiPSCs were transduced with a lentiviral vector encoding inducible FLP (FLP ERT2 ) under the control of the MAB21L2 promoter at day 3 and were treated with 4-OHT at days 7 and 8 before analysis at day 12.
#Workflow#Illustration#JCF cells#Epicardioids#AAVS1-FRT#STOP-mKate2-HA reporter hiPSCs#Lentiviral vector#FLP ERT2#MAB21L2 promoter#4-OHT
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