Microscopy from Scientific Research

MIPs of neutrophils with high-speed migration in a living mouse liver, obtained by LFM, VsLFM and sLFM, respectively. The neutrophil at t = 86.3 s moved slowly without motion artifacts, and the retraction fiber could be observed with high resolution by both VsLFM and sLFM. However, at t = 488.8 s the neutrophil migrated at high speed, leading to visible motion artifacts in the sLFM results. The upper-right insets show the corresponding Fourier transform of the MIPs, which also show the periodic frequency patterns caused by sample motion in the sLFM results. Meanwhile, VsLFM effectively eliminates motion artifacts with high spatial resolution. Scale bars, 10 μm ( a c ), 10 μm (main) and 3 μm -1 (inset).

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