Bar Plot from Scientific Research

Basal brightness of NEMO, NCaMP7 or GCaMP6m sensors viewed with YFP (top) or GFP (bottom) filters. To achieve better estimation of the basal fluorescence of GECIs ( F GECI ), F GECI of cells expressing mKate-P2A-GECI constructs was normalized against the fluorescence of mKate, an expression marker ( F mKate ). (GCaMP6m, n = 99 cells; NEMOm, n = 142 cells; NEMOb, n = 119 cells NEMOc, n = 89 cells; NEMOs, n = 91 cells; NEMOf, n = 86 cells; NCaMP7, n = 114 cells). Three independent biological repeats. Data are shown as mean ± s.e.m.

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