Activation of pPDGFRβ (phosphorylation of Y751) in the presence of Nsp3(EEEV) in HEK293-PDGFRβ-HA cells probed by PLA. Constructs used are color coded as in ( C ). Results were quantified as in ( C ) (no transfection: N = 3029, 3149, and 3134 at 0, 10, and 60 min, respectively; mock transfection: N = 872, 923, and 1074 at 0, 10, and 60 min, respectively; Nsp3 (EEEV) wt: N = 1203, 1174, and 1365 at 0, 10, and 60 min, respectively, Nsp3 (EEEV) mut: N = 1086, 1215, and 1225 at 0, 10, and 60 min, respectively) over three (mock transfection) or six (Nsp3 (EEEV) wt, Nsp3 (EEEV) mut and no transfection) biological replicates. Significance was determined as in ( C ) and asterix denote same statistical significance as in ( C ). Corresponding fluorescence microscopy images for ( C ) and ( I ) are shown in Figure S8 and Figure S11 .
