The interaction between clathrin and full-length Nsp3 (EEEV) in HEK293 cells probed by proximity ligation assay (PLA). The results show PLA signal per cell (no transfection: N = 3270, mock transfection N = 1664, Nsp3 (EEEV) wt: N = 1075, Nsp3 (EEEV) mut: N = 1007) over six biological replicates and are presented as violin plots with indicated median and interquartile range. P (HeV) mut was used as a mock transfection. Significance was determined by Kruskal–Wallis rank sum test with two-sided Dunn’s test and Bonferroni correction as a post hoc test to compare all groups; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Source data for all PLA experiments are provided in the Source data file.
