Quantification of the retention of activated PDGFRβ at the plasma membrane as observed by cell surface fluorescence assay. Constructs used are color coded as in ( C ). Integrated fluorescence intensity was measured over 3 biological replicates (no transfection: N = 1940 and 1925 at 0 and 60 min, respectively; mock transfection: N = 586 and 1058 at 0 and 60 min, respectively; Nsp3 (EEEV) wt: N = 380 and 419 at 0 and 60 min, respectively, Nsp3 (EEEV) mut: N = 509 and 453 at 0 and 60 min, respectively). Two-sided Wilcoxon rank sum test with Bonferroni correction for pairwise comparison was used to analyze statistical variance. Asterix denote same statistical significance as in ( C ). Corresponding fluorescence microscopy images are shown in Figure S12 .
