Superimposition of the experimental temperature coefficient changes between 30 C and 50 C and the asymmetric dynamical perturbation contact network between all backbone NH moieties and the rest of the protein. The absolute variation between temperature coefficient around 30 C and 50 C are displayed in green spheres centered on the nitrogen atoms of N-H groups, with sphere sizes being proportional to the variation in the slope (ppb/K). Gray spheres refer to residues that could not be assigned unambiguously over the full temperature range in HisF, thus missing temperature coefficient values. In the perturbation network, blue and red edges represent a decrease and increase, respectively, of contacts upon an increase in temperature. Edge widths are proportional to the differences in number of contacts.
