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Immuno-FISH staining in WT and EXD2 -/- U2OS synchronised prometaphase cells treated with siRNA targeting BLM, SLX4, SMARCAL1 or control siRNA. EdU incorporation was determined by Click-iT staining (red) and telomeres marked by a telomere-specific PNA FISH probe (TelG, green), DAPI (blue) acts as a nuclear stain ( n = 80, 80, 76, 75, 55, 55, 51, 42, 54, 56, 62 and 48 prometaphases examined over 2 independent experiments, statistical significance was determined by two-sided MannWhitney analysis, bars represent +/- SEM, scale bar = 10 m).

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