Heatmap from Scientific Research

Left, to assess the cross-reactivity of each designed peptide binder in Fig. 2 with each target peptide, biotinylated target peptides were loaded onto bio-layer interferometry streptavidin sensors and allowed to equilibrate, and the baseline signal was set to zero. The bio-layer interferometry tips were then placed into a solution containing proteins at the indicated concentrations for 500 s and washed with buffer, and dissociation was monitored for another 500 s. The heat map shows the maximum signal for each binder–target pair (cognate and non-cognate) normalized by the maximum signal of the cognate designed binder–target pair. Right, surface shape complementarity of the cognate complexes. The peptides are in sphere representation.

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